Columns
The 4 µm, 80 Å (80 Å) silica support provides high chromatographic efficiency, while the very high surface area material (500 m2/g) offers exceptional interactions.This combination provides excellent peak symmetry.
The J'sphere (TM) series combines three different carbon loads-9%, 14% and 22%-with a range of hydrogen-bonding capacities to provide three columns that differ in retention and often in selectivity as well.You can exploit these differences in method development without ever leaving the quality of product and support chromatographers have come to expect from YMC.
ODS-H80, with 22% carbon is the most hydrophobic and hydrolytically stable. It resists attack by both high- and low-pH solvents and has proven stable at pH's 1.1 to 9.3 for over 500 hours!
ODS-M80, with 14% carbon, is an inert, efficient phase with moderate hydrophobicity and hydrogen bonding capacity.
ODS-L80, with 9% carbon offers retention similar to C8, but with unique selectivity imparted by its high hydrogen-bonding capacity.
These three columns afford the chromatogapher a range of retention on a pure, high-efficiency, reproducible support.
| SPECIFICATION | |
| Particle size | 4 µm |
|---|---|
| Pore size | 80 Å |
| Carbon contents | ODS-H80 22% ODS-M80 14% ODS-L80 9% |
| Usable pH range | ODS-H80 1-9 ODS-M80/ODS-L80 2-7.5 |
Three different types of ODS with different ligand coverage display different hydrophobicity and hydrogen bonding capacities and steric selectivity. ODS-H80 has higher hydrophobicity and less hydrogen bonding capacity. ODS-M80 has moderate hydrophobicity and hydrogen bonding capacity. ODS-L80 has less hydrophobicity similar to a C8 and high hydrogen-bonding capacity.
| Column | 150 X 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/water (75/25) |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 254 nm |
| Column | 150 X 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/20 mM KH2PO4 (15/85) |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 254 nm |
ODS-H80 shows acid resistance and alkali resistance. It is effective for separation of peptides and basic compounds.
| Column | J'sphere ODS-H80, 150 X 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/water (60/40) |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 270 nm |
| Sample | butyl benzoate |
| Column | J'sphere ODS-H80, 150 X 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/water (60/40) |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 270 nm |
| Sample | butyl benzoate |
