C8 is the most commonly used column type following C18. YMC Octyl columns are prepared from exhaustive bonding of a monomeric octylsilane to totally spherical and porous silica gels. The functionalized silica is then treated with a endcapping process. This produces a moderate 10% carbon loading on the standard 120 Å pore material.
C8 columns are well suited for the separation of many compounds which are too strongly retained on C18 phases or require greater retention than C4. It is the most versatile reversed phase column and should be considered a first choice when outfitting a new laboratory.
Available in three pore size, YMC Octyl columns will separate many classes of compounds including pharmaceuticals, organic chemicals and biologicals.
For preparative applications, choose the smallest pore size which provides adequate retention and resolution. This is because sample loading is generally proportional to surface area. Small pore packings provide greater surface area.
SPECIFICATION | |
Particle size | 3 µm, 5 µm, 10 µm |
---|---|
Pore size | 120 Å, 200 Å, 300 Å |
Carbon contents | 10%, 7%, 4% |
Usable pH range | 2-7.5 |