Columns
The YMC Diol-NP stationary phase is a versatile alternative to silica for normal phase separations. The bonded layer's-OH groups provide good selectivity without excessive retention, since hydrogen bonding with the Diol-NP layer is not as strong as one with the silanols on a bare silica surface.
Diol-NP columns also provide better reproducibility when compared with bare silica columns.
Like all YMC silica-based bonded phase,the Diol-NP phase starts with an underlying silica support of exceptional purity.
YMC manufacturing and quality assurance possession ensure that the silica has a very low metal content. The silica purity greatly reduces nonspecific sample adsorption. As a result, you can expect excellent sample recovery.
The high surface area of the underlying silica particle, together with the larger number of available sites for interaction of the 1, 2 dihydroxypropane ligands, results in good preparative loading.
| SPECIFICATION | |
| Particle size | 5 µm |
|---|---|
| Pore size | 60 Å, 120 Å |
| Usable pH range | 2-7.5 |
This figure shows differences in selectivity of phenols when separated on Diol-NP and SIL under the same conditions. For these compounds, Diol columns provide longer retention time and better separation.
| Column | 250 X 4.6 mmI.D. |
|---|---|
| Eluent | n-hexane/ethanol (80/20) |
| Flow rate | 1.0 mL/min |
| Temperature | 30°C |
| Detection | UV at 254 nm |
