Columns
All phases in the Pro-family are ideal for the analysis of all types of organic molecules but specifically basic and metal chelating compounds. The ultrapure silica and very low residual silanol activity means that these columns frequently do not require the use of ion-pairing reagents or ion-suppression techniques, making them ideal for LC/MS applications.
| SPECIFICATION | |
| Particle size | 3 µm, 5 µm |
|---|---|
| Pore size | 120 Å |
| Carbon contents | C8--10% C4--7% |
| Usable pH range | 2-7.5 |
Effectively endcapped Pro C8 and C4 offer better peak shape for separation of basic compounds such as alkaloids.
| Column | YMC-Pack Pro C8 (5 µm, 120 Å) 150 x 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/20 mM KH2PO4 (20/80) |
| Flow rate | 1.0 mL/min |
| Temperature | 30°C |
| Detection | UV at 210 nm |
This figure shows that Pro C8 and C4 columns allow us to minimize total analysis time while maintaining excellent separation for caffeine and ethenzamide and bromovalerylurea.
| Column | 150 x 4.6 mmI.D. |
|---|---|
| Eluent | methanol/20 mM H3PO4 (60/40) |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 254 nm |
In the case that it is difficult to optimize resolution and analysis time on separation by C18, C8 and C4 columns can be effective alternatives.
| Column | 150 x 4.6 mmI.D. |
|---|---|
| Eluent | 20 mM KH2PO4-K2HPO4 (pH6.9) /methanol |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 220 nm |
