YMC-Triart C8 is a versatile column with excellent chemical durability that is equivalent to YMC-Triart C18. YMC-Triart C8 is suitable for fast analysis of samples containing hydrophobic compounds that are strongly retained on C18 columns or samples containing compounds with large differences in hydrophobicity. In addition, its high bonding density allows for good separation of compounds with small structural differences. Triart C8 is also ideal for the separation of isomers and structural analogs.
SPECIFICATION | |
Base | organic/inorganic hybrid silica |
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Particle size | 1.9 µm, 3 µm, 5 µm |
Pore size | 120 Å |
Carbon contents | 17% |
pH range | 1-12 |
YMC-Triart C8 has good chemical durability and provides peak shapes as good as YMC-Triart C18. It is useful applications in various fields including pharmaceutical products, food products, and natural products.
Column | YMC-Triart C8 5 µm 150 X 4.0 mmI.D. |
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Eluent | A) phosphate buffer (pH 6.7)*/acetonitrile (93/7) B) acetonitrile 0-8%B (0-12 min), 8-62%B (12-28 min) * Dissolve 1.25 g of KH2PO4 and 2.01 g of Na2HPO4・12H2O in 1000 mL of water |
Flow rate | 1.0 mL/min |
Temperature | 35°C |
Detection | UV at 280 nm |
Injection | 20 µL |
(The United States Pharmacopeia 34th; Assay) |
Triart C8 provides superior resolution of Terphenyl isomers compared to Triart C18. The higher bonding density of C8 contributes to the recognition of small differences in structure although the elution profiles of C18 and C8 are similar. Additionally, the C8 phase offers shorter retention times than the C18 phase due to its low hydrophobicity. These unique characteristics are effective for the fast analysis of isomers and compounds with low polarity.
Column | 5 µm, 150 X 3.0 mmI.D. |
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Eluent | methanol/water (75/25) |
Flow rate | 0.425 mL/min |
Temperature | 30°C |
Detection | UV at 254 nm |