Columns
Phenyl columns provide unique selectivity when compared to aliphatic straight chain reversed phases such as ODS, C8 and C4. The π electrons of the phenyl group can interact with the aromatic ring of an analyte to increase retention relative to non-aromatic species. Phenyl also has application as an alternative to ODS and C4, for the analysis of peptides and proteins, on both small pore 120Å and wide-pore 300Å silica supports. Retention is decreased on wide-pore phenyl phases relative to 120Å phenyl material. Generally, as pore size increases surface area decreases, resulting in shorter retention times.
Particle size | : 3µm, 5µm |
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Pore size | : 120Å |
Carbon contents | : 9% |
Usable pH range | : 2.0-7.5 |
Column | : 150 x 4.6mmI.D. |
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Eluent | : acetonitrile /50mM NH4H2PO4 (10/90) |
Flow rate | : 1.0 mL/min |
Temperature | : 37ºC |
Detection | : UV at 254 nm |
Injection | : 10µL (0.02-0.30mg/mL) |
This figure shows difference in retention behavior of Ph and ODS for separation of water-soluble vitamins.