Columns
BioPro HIC columns are capable of separating proteins under non-denaturing conditions. BioPro HIC HT offers a time-critical analysis of biopharmaceuticals such as antibody-drug conjugates (ADCs). BioPro HIC BF is useful for separation of especially low hydrophobic proteins.
| Matrix | Bonded phase |
Particle size (µm) |
Usable pH range |
Usable temperature range |
Characteristics | |
|---|---|---|---|---|---|---|
| BioPro HIC HT | Hydrophilic non-porous polymer |
Butyl group | 2.3 | 2-12 | 10-60°C | First-choice HIC column for high resolution/high throughput analyses |
| BioPro HIC BF | 4 | HIC column for separation of low hydrophobic proteins |
BioPro HIC HT (first choice column) is suitable for successful separation of various biopharmaceuticals.
BioPro HIC BF shows the stronger retention of proteins than BioPro HIC HT due to the stationary phase hydrophobicity, and could be preferred for the separation of low hydrophobic proteins.
| Column | 100 X 4.6 mmI.D. |
|---|---|
| Eluent | A) 100 mM NaH2PO4-Na2HPO4 containing 2.0 M (NH4)2SO4 (pH 7.0) |
| Flow rate | 0.5 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 15 µL |
| Sample | 1. Adalimumab (0.5 mg/mL) |
