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Silica based packing materials
YMC*GEL/YMC*GEL HG

YMC*GEL for preparative HPLC has various available functional groups, such as reversed-phase and normal-phase, and also various particle sizes from 3 µm with high-resolution to 150 µm for open column chromatography. YMC*GEL is able to work for a long period of time without particle breakage, as it uses high-density and high-strength silica gel. YMC*GEL/YMC*GEL HG packing materials are widely used both domestically and internationally.

POINTS
  • Packing materials with various functional groups and particle sizes are available
  • High density and high mechanical strength silica gel (YMC*GEL HG)
  • Suited for large-scale purification by using dynamic axial compression column
  • Packing material for open column chromatography is available
Features

Excellent mechanical stability (YMC*GEL HG)

The high mechanical stability of YMC*GEL HG is demonstrated by means of repeated measurements with a dynamic axial compression column (DAC). Even after more than 10 repacking cycles with the same material, the pressure does not increase. The absence of fines is proven by a constant backpressure.

Packing conditions
Column size 100 X 50 mmI.D.
Packing pressure 6.5 MPa
Test conditions
Eluent methanol/water（85/15）
Flow rate 50 mL/min

Suitable for separation of peptides

YMC*GEL/YMC*GEL HG are available with various functional groups and also with wide pores. Therefore, this material is suitable for the preparation of peptides, such as insulin and proteins. YMC*GEL HG can make scaling up from analytical scale to preparative scale easy, as it has the same performance as that of YMC-Pack.

Analytical condition (1), (3)
Column YMC-Pack C8 (5 µm, 200 Å)
150 X 4.6 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 0.8 mL/min
Temperature ambient
Detection UV at 220 nm
Preparative condition (2)
Column YMC*GEL C8 (10 µm, 200 Å)

150 X 50 mmI.D.
Eluent A) water/TFA (100/0.1)
B) acetonitrile/TFA (100/0.1)
27%B (0-5 min)
27-40%B (5-20 min)
Flow rate 100 mL/min
Temperature ambient
Detection UV at 220 nm

Lineup

YMC＊GEL/YMC＊GEL HG

Packing material Particle size
(µm)
Pore size
(Å)		 Characteristics
ODS-A 3, 5, 75, 150 120, 200, 300
  • Standard ODS
ODS-A-HG 7, 10, 15, 20, 50
ODS-AM 3, 5 120
  • Outstanding lot-to-lot reproducibility
ODS-AQ 3, 5 120, 200
  • Superior separation of hydrophilic compounds
ODS-AQ-HG 7, 10, 15, 20, 50
C8 3, 5 120, 200, 300
  • Useful for separation of relatively highly hydrophobic compounds
  • Useful for separation of proteins and peptides
C8-HG 10, 15, 20, 50
C4 3, 5 120, 200, 300
  • Stationary phase with low hydrophobicity
  • Useful for separation of proteins and peptides
C4-HG 10, 15, 20, 50
TMS 3, 5 120
  • Reverse phased packing material with the lowest hydrophobicity
TMS-HG 10, 15, 20, 50
Ph(Phenyl) 3, 5 120
  • Different separation characteristics from C18
Ph-HG 10, 15, 20, 50
CN 3, 5 120
  • The medium polarity of the functional group allows selectable normal-phase and reversed-phase separation modes
CN-HG 10, 15, 20, 50
SIL 3, 5, 75, 150 60, 120, 200, 300
  • Standard of normal-phase packing material
SIL-HG 10, 15, 20, 50
Diol 3, 5 60, 120, 200, 300
  • Useful for gel filtration or normal-phase applications
Diol-HG 10, 15, 20, 50
NH2 5 120
  • Chemically bonded with aminopropyl groups
NH2-HG 10, 15, 20, 50

Packing materials for Open column chromatography

Packing material Particle size
(µm)
Pore size
(Å)
ODS-A 75, 150 120
SIL 75, 150 120
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