Bulk packings
YMC-Triart and YMC-Triart Prep are bulk packing materials based on novel organic/inorganic hybrid silica particles. These materials can be widely applied from lab-scale purification to industrial production. Their excellent durability allows effective cleaning of the gel with alkaline solution. These materials provide excellent mechanical stability, and can be packed into a column repeatedly. The longer lifetime of YMC-Triart Prep greatly contributes to the reduction of production cost.
| Product | Particle size (µm) |
Pore size (Å) |
Carbon content * (%) |
Usable pH range |
|---|---|---|---|---|
| Triart SIL | 3, 5 | 120 | - | - |
| Triart C18 | 20 | 1-12 | ||
| Triart C8 | 17 | |||
| Triart Prep SIL | 7, 10, 15, 20 | - | - | |
| Triart Prep C18-S | 20 | 2-10 | ||
| Triart Prep C8-S | 10, 15, 20 | 17 | ||
| Triart Prep C4-S | 10 | 14 | ||
| Triart Prep Phenyl-S | 17 | |||
Triart Prep Bio200 C8 |
200 | 14 |
*Containing carbon content for hybrid silica base material.
YMC-Triart is based on novel organic/inorganic hybrid particles. The particles combine the high mechanical stability and high efficiency of silica-based packing materials with the high chemical stability of polymer-based packing materials. A granulation process utilizing microreactor technology enables continuous and highly controlled production of the hybrid particles. The particles have uniform pore size distributions and smooth surfaces, as well as uniform particle sizes. This feature greatly contributes to excellent peak shape and separation reproducibility.
YMC-Triart Prep materials are highly acid-proof at lower pH conditions and alkaline-proof at higher pH conditions. These features enable purification with a mobile phase containing TFA and cleaning with alkaline solution, which are often used for peptide and protein purification.
| Column | 10 µm, 120 Å, 250 X 4.6 mmI.D. |
|---|---|
| Eluent | acetonitrile/water (60/40) |
| Flow rate | 1.0 mL/min |
| Temperature | 37°C |
| Detection | UV at 254 nm |
| Sample | n-butyl benzoate |
| Column | 10 µm, 120 Å, 150 X 4.6 mmI.D. |
|---|---|
| Eluent | 50 mM triethylamine in methanol/ 50 mM triethylamine in water (20/80) |
| Flow rate | 1.0 mL/min |
| Temperature | 50°C |
| Detection | UV at 254 nm |
| Sample | caffeine |
Repeated injections of a sample may induce adsorption of proteins. This would result in loss of retention of a target and/or resolution. It is effective to use cleaning with an alkaline solution. Triart Prep with outstanding alkaline stability provides longer lifetime even with a process that includes such chemically harsh washing.
Test procedure
| Column | 50 X 4.6 mmI.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile 26-36%B (0-3 min), 36%B (3-4 min), 26%B (4-7 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 30 µL |
| Sample | insulin (10 mg/mL) |
Triart Prep has high mechanical stability. Even if it is packed and unpacked repeatedly in a dynamic compression column, no particle crashes and/or pressure building-up are observed.
Change in column pressure
| Column packing | |
| Packing material | YMC-Triart Prep Bio200 C8 (10 µm, 200 Å) |
|---|---|
| Column size | 100 X 50 mmI.D. |
| Packing pressure | 6.5 MPa | Pressure measurement |
| Eluent | methanol/water (85/15) |
| Flow rate | 50 mL/min |
| Temperature | ambient |
SEM images
Triart Prep C18-S materials are designed to have identical separation characteristics to analytical Triart C18 material. Furthermore, Triart Prep C18-S materials give exactly the same separation behavior among particle sizes. A method can be seamlessly transferred between particle sizes as well as between Triart C18 and Triart Prep C18-S.
| Column | 250 X 4.6 mmI.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 20-40%B (0-45 min) |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 220 nm |
| Injection | 10 µL (0.167 mg/mL) |
