Columns
YMC-SEC MAB is a silica-based size exclusion chromatography (SEC)/gel filtration chromatography (GFC) column with excellent durability and batch-to-batch reproducibility. Optimized base material design and surface modification provide superior separation of antibody monomers and their aggregates/fragments with very low nonspecific adsorption. This is ideal for the quality control assessment of antibody drugs.
| Specification | |
| Base | Silica gel |
|---|---|
| Particle size | 3 µm |
| Pore size | 250 Å |
| Bonded phase | Dihydroxypropyl |
| Usable pH range | 5-7.5 |
| Molecular weight range | 10,000-700,000 |
A humanized monoclonal antibody and an ADC were analyzed using YMC-SEC MAB. Chromatograms show excellent peak shape, and good separation between aggregates and a monomer peak. YMC-SEC MAB is effective for characterization and quality control of antibody drugs.
Analysis of a humanized monoclonal antibody
| Column | YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D. |
|---|---|
| Eluent | 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl |
| Flow rate | 0.165 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 10 µL |
| Sample | Humanized monoclonal antibody (5 mg/mL) |
Analysis of an antibody-drug conjugate (ADC)
| Column | YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D. |
|---|---|
| Eluent | 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl/2-propanol (85/15) |
| Flow rate | 0.165 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 4 µL |
| Sample | Cystein-conjugated ADC mimic (2.5 mg/mL) |
YMC-SEC MAB has excellent repeatability of separation without any change of theoretical plate number and elution time even after repeated separation of a monoclonal antibody for more than 500 times because of its superior durability.
| Column | YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D. |
|---|---|
| Eluent | 0.1 M NaH2PO4-Na2HPO4 (pH 7.0) containing 0.2 M NaCl |
| Flow rate | 0.33 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 2 µL |
| Sample | Humanized monoclonal antibody (1 mg/mL) |
YMC-SEC MAB provides excellent reproducibility of the separation of monomer and aggregates as well as for monomer and fragments. Therefore, it is very effective for quality control of antibody drugs.
| Column | YMC-SEC MAB (3 µm, 250 Å) 300 X 4.6 mmI.D. |
|---|---|
| Eluent | 0.1 M KH2PO4-K2HPO4 (pH 7.0) containing 0.2 M NaCl |
| Flow rate | 0.165 mL/min |
| Temperature | 25°C |
| Detection | UV at 280 nm |
| Injection | 10 µL |
| Sample | Humanized monoclonal antibody (5 mg/mL) |
