Columns
YMC-Triart Bio C18, which is a wide-pore column with organic/inorganic hybrid silica, is suitable for separation of peptides, proteins and oligonucleotides. YMC-Triart Bio C18 is also possible to analyze sample materials in a short time, which are strongly retained on conventional C18 columns, due to its low hydrophobicity.
| SPECIFICATION | |
| Base | organic/inorganic hybrid silica |
|---|---|
| Particle size | 1.9 µm, 3 µm, 5 µm |
| Pore size | 300 Å |
| Usable pH range | 1-12 |
| USP | L1 |
The widths at half the peak height (WHPH) were compared when peptides and proteins with molecular weight (MW) from 1,859 to 76,000 were separated using Triart Bio C18 and Triart Bio C4 with pore size 300 Å, and Triat C18 with pore size 120 Å. Good peak shape was obtained using Triart Bio C18 and Triart Bio C4, which are wide-pore columns, even in separation of proteins with high MW. On the other hand, peak broadening was observed when using Triart C18 (120 Å) on separation of proteins with MW over 10,000. Wide-pore columns with pore size 300 Å are suitable for separation of proteins with MW over 10,000.
| Column | 150 X 3.0 mmI.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) |
| Flow rate | 0.425 mL/min |
| Temperature | 40ºC |
| Detection | UV at 220 nm |
| Injection | 4 µL (0.1-0.5 mg/mL) |
| Sample | γ-Endorphin (MW 1,859), Insulin (MW 5,733), |
This example shows that chromatograms of Human growth hormone, a peptide with MW 22,000, which was separated using Triart Bio C18 and Triart Bio C4 with pore size 300 Å, and Triart C18 with pore size 120 Å, respectively. Good peak shape can be obtained on analysis of high MW molecules such as proteins with Triart Bio C18 and Triart Bio C4, wide-pore columns. Excellent separation was achieved using Triart Bio C18 with longer alkyl chain in its bonded phase.
| Column | 150 X 3.0 mmI.D. |
|---|---|
| Eluent | A) water/TFA (100/0.1) |
| Flow rate | 0.425 mL/min |
| Temperature | 40ºC |
| Detection | UV at 220 nm |
| Injection | 8 µL |
| Sample | Human growth hormone (0.1 mg/mL) |
Triat Bio C18 is suitable for highly sensitive analysis and structural analysis of proteins using LC-MS since it gives good peak shape in mobile phase containing formic acid.
| Column | 150 X 3.0 mmI.D., 150 X 4.6 mmI.D. |
|---|---|
| Eluent | A) water/formic acid (100/0.1) |
| Flow rate | 0.425 mL/min for 3.0 mmI.D. |
| Temperature | 40ºC |
| Detection | UV at 220 nm |
| Sample | Human growth hormone (0.1 mg/mL) |
