Columns
YMC-Triart series 1.9 µm columns based on novel, multi-layered, organic/inorganic hybrid silica particles are designed for UHPLC. The outstanding mechanical strength of Triart series 1.9 µm makes it ideal for use at higher pressures typical of UHPLC.
YMC-Triart series 1.9 µm offers high resolution with short length column. Since its efficiency does not decrease even if the flow rate increases, it enables ultra high throughput analysis.
Ultra-fast separation of drugs
| Eluent | A) 10 mM CH3COONH4-CH3COOH (pH 5.5) B) acetonitrile |
|---|---|
| Detection | UV at 254 nm |
| Temperature | 30℃ |
| Injection | 2 µL |
YMC-Triart series 1.9 µm has superior column efficiency. It is suitable for multi-component separation such as peptide mapping that requires high resolution.
Peptide mapping
| Column | YMC-Triart C18 |
|---|---|
| Eluent | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.08) 5-40%B (0-15 min) for a single column 5-40%B (0-30 min) for two coupled columns |
| Flow rate | 0.4 mL/min |
| Temperature | 70℃ |
| Detection | UV at 220 nm |
| Injection | 10 µL for a single column 20 µL for two coupled columns |
| Sample | Tryptic digest of Bovine Hemoglobin |
| System | Agilent 1290 |
YMC-Triart series 1.9 µm's innovative surface modification technology results in excellent peak shapes even for the basic and coordination compounds that often exhibit adsorption or tailing shapes on other UHPLC columns. Thus, its quantitative capability is sufficient, and decreases the risk of missing unknown constituents. YMC-Triart series stands head and shoulders above any other columns in quantitative capability.
Comparison of chromatographic performance for ionic compounds with UHPLC column
| Basic compounds | |
| Column | 50 X 2.0 mmI.D. or 50 X 2.1 mmI.D. |
|---|---|
| Eluent | 20 mM KH2PO4-K2HPO4 (pH 6.9)/acetonitrile (65/35) |
| Flow rate | 0.2 mL/min |
| Temperature | 40℃ |
| Detection | UV at 235 nm |
| Sample | 1. Chlorpheniramine 2. Dextromethorphan 3. Propyl paraben (I.S.) |
| Coordination compound | |
| Column | 50 X 2.0 mmI.D. or 50 X 2.1 mmI.D. |
|---|---|
| Eluent | acetonitrile/0.1% H3PO4 (40/60) |
| Flow rate | 0.2 mL/min |
| Temperature | 40℃ |
| Detection | UV at 254 nm |
| Sample | 1. Hinokitiol 2. Methyl benzoate (I.S.) |
