Columns
YMC-Triart SIL column packed with bare organic/inorganic hybrid silica particles is intended for use in normal-phase and HILIC modes. It is more rugged than conventional silica columns due to the chemical stability of the hybrid silica particles.
| SPECIFICATION | |
| Base | organic/inorganic hybrid silica |
|---|---|
| Particle size | 3 µm, 5 µm |
| Pore size | 120 Å |
| Usable pH range | 2-8 |
| USP | L3 |
The amidite reagents, which is a raw material for nucleic acid medicine that have been attracting attention in recent years, were analyzed using Triart SIL. Triart SIL has small surface polarity and low nonspecific adsorption because it is organic/inorganic hybrid silica. Therefore, it shows good peak shape compared to conventional inorganic silica gel column.
| Column | 5 µm, 120 Å 250 X 4.6 mmI.D. |
|---|---|
| Eluent | n-hexane/ethyl acetate/triethylamine (50/50/0.1) for dT n-hexane/ethyl acetate/triethylamine (25/75/0.1) for dG |
| Flow rate | 1.0 mL/min |
| Temperature | 25°C |
| Detection | UV at 285 nm |
In analytical scale (inner diameter 4.6 mm) , Triart SIL column was able to load 20 mg of amidite reagent. It means that approximately 380 mg be able to load with an inner diameter of 20 mm, and approximately 850 mg be able to load with an inner diameter of 30 mm.
Good peak shape of Triart SIL offers high loading and high purity purification.
| Column | YMC-Triat SIL (5 µm, 120 Å) 250 X 4.6 mmI.D. |
|---|---|
| Eluent | n-hexane/ethyl acetate/triethylamine (50/50/0.1) |
| Flow rate | 1.0 mL/min |
| Temperature | ambient |
| Detection | UV at 285 nm |
| Sample | DMT-dT-CE Phosphoramidite |
Cetirizine hydrochloride
| Column | YMC-Triart SIL (5 µm, 120 Å) 250 X 4.0 mmI.D. |
|---|---|
| Eluent | acetonitrile/0.04 M H2SO4 (94/6) |
| Flow rate | 0.75 mL/min |
| Temperature | 25°C |
| Detection | UV at 230 nm |
| (The Japanese Pharmacopoeia 17th; Purity (2) Related substances) | |
Shipping solvent is n-hexane/2-propanol (99.5/0.5). In case of using eluent including water, take care of miscibility.
