カラム
| 種類 | pKa | Buffer range | 一般的な使用濃度 | 揮発性 （LC/MSや分取への適応） |
| TFA | ＜1.0 | 0.01～0.1% | ○ | |
| H3PO4 | 2.1 | 0.01～0.1% | × | |
| NH4H2PO4-H3PO4 [NaH2PO4-H3PO4, KH2PO4-H3PO4] |
2.1 | 1.1～3.1 | 5～50 mM （＜20 mM推奨） |
× |
| HCOOH | 3.7 | 0.1～1.0% | ○ | |
| HCOONH4-HCOOH [HCOONa-HCOOH, HCOOK-HCOOH] |
3.7 | 2.7～4.7 | 5～50 mM | NH4 salt：○ [Na, K salt：×] |
| CH3COOH | 4.8 | 0.5～5.0% | ○ | |
| CH3COONH4-CH3COOH [CH3COONa-CH3COOH, CH3COOK-CH3COOH] |
4.8 | 3.8～5.8 | 5～50 mM | NH4 salt：○ [Na, K salt：×] |
| (NH4)2HPO4 -NaH2PO4 [Na2HPO4-NaH2PO4, K2HPO4-KH2PO4] |
7.2 | 6.2～8.2 | 5～50 mM （＜20 mM推奨） |
× |
| Triethylamine-CH3COOH (TEAA) | 4.6～6, 10～11 | ＜20 mM | ○ | |
| HCOONH4-NH3, CH3COONH4-NH3 | 9.2 | 8.2～10.2 | ＜20 mM | ○ |
| Na3PO4-Na2HPO4, K3HPO4-K2HPO4 | 12.3 | 11.3～13.3 | ＜10 mM | × |
| NH4HCO3-NH3 | 8.5～10.5 | ＜10 mM | ○ |
