CHIRAL ART have outstanding packed bed stability provided by the use of high-strength super-wide-pore silica and innovative packing technology. The column efficiency and selectivity are maintained, even after sequential gradient tests at a high flow rate (three times higher than the normal flow rate) and a high pressure (rapid pressure change).
CHIRAL ART are suitable for the case where one intends to shorten an analysis time, (re-)equilibration time, and/or column cleaning time by increasing the flow rate. This feature is also effective when a highly viscous solvent needs to be used as a mobile phase on immobilized type columns.
Sequential gradient test | |
Column | 5 µm, 250 X 4.6 mmI.D. |
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Eluent | A) n-hexane, B) ethanol 0-100%B (0-15 min) |
Flow rate | 3.0 mL/min |
Pressure | 10-30 MPa/run |
Temperature | 37℃ |
Column performance test | |
Column | 5 µm, 250 X 4.6 mmI.D. |
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Eluent | n-hexane/ethanol (90/10) |
Flow rate | 1.0 mL/min |
Temperature | 37℃ |
Detection | UV at 230 nm |
Sample | trans-Stilbene oxide |
On both analytical and preparative separation, separation behavior of CHIRAL ART is equivalent to that of the competitor’s product.
Column | 5 µm, 250 X 4.6 mmI.D. |
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Eluent | n-hexane/ethanol (90/10) |
Flow rate | 1.0 mL/min |