Columns

CHIRAL ART Immobilized type

Features

Excellent peak shape

CHIRAL ART provide good peak shapes for ionic and metal coordination compounds.

Column 5 µm, 250 X 4.6 mmI.D.
Eluent n-hexane/2-propanol/diethylamine
(80/20/0.1)
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 230 nm
Injection 10 µL (0.1 mg/mL)

Low column bleeding

CHIRAL ART Immobilized type show remarkably reduced background signals under typical gradient conditions. The low column bleeding of these columns provides high sensitivity for LC/MS analysis owing to the very low ion suppression and stable baseline. CHIRAL ART Immobilized type offers excellent robustness for gradient analysis and highly sensitive analysis on LC/MS.

Gradient test
Column 5 µm, 250 X 4.6 mmI.D.
Eluent A) n-hexane
B) ethanol
2-80%B (0-30 min)
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 230 nm

Wide usable pH range

CHIRAL ART Immobilized type has excellent chemical stability and can be used across a wide pH range. The addition of acid/amine to the mobile phase or the use of buffer solution is an effective tool for improving peak shape and/or resolution.

Continuous flow of acid/alkaline solution
Column 5 µm, 50 X 4.6 mmI.D.
Eluent Buffer/methanol (90/10)
Flow rate 1.0 mL/min
【Acidic condition】
Buffer 0.1% H3PO4 (pH 2)
Temperature 40°C
【Basic condition】
Buffer 20 mM NH4HCO3-DEA (pH 9)
Temperature 25°C
Column performance test
Column 5 µm, 50 X 4.6 mmI.D.
Eluent acetonitrile/water (30/70) for SB
methanol/water (70/30) for SJ
Flow rate 1.0 mL/min
Temperature 25°C
Detection UV at 254 nm
Sample Benzoin for SB
1-(1-Naphthyl) ethanol for SJ

High solvent versatility

On CHIRAL ART Immobilized type, the change in column performance after flushing with each solvent was less than 2%. Thus, these columns have high resistance to various solvents.

Retention rate of initial column performance (after flushing with 1,000CV of each solvent at 40°C)  *CV=Column Volume
  Amylose-SA Cellulose-SB Cellulose-SJ
α k'(2) α k'(2) α k'(2)
Ethyl acetate 100.3% 101.2% 100.0% 99.1% 99.3% 99.0%
Tetrahydrofuran 100.0% 100.0% 99.3% 98.0% 99.2% 99.7%
Dichloromethane 100.3% 100.6% 101.3% 99.6% 99.6% 98.4%
Column 5 µm, 50 X 4.6 mmI.D.
Eluent n-hexane/2-propanol (95/5)
Flow rate 1.0 mL/min
Temperature 25°C
Sample Benzoin