CHIRAL ART provide good peak shapes for ionic and metal coordination compounds.
Column | 5 µm, 250 X 4.6 mmI.D. |
---|---|
Eluent | n-hexane/2-propanol/diethylamine (80/20/0.1) |
Flow rate | 1.0 mL/min |
Temperature | 25°C |
Detection | UV at 230 nm |
Injection | 10 µL (0.1 mg/mL) |
CHIRAL ART Immobilized type show remarkably reduced background signals under typical gradient conditions. The low column bleeding of these columns provides high sensitivity for LC/MS analysis owing to the very low ion suppression and stable baseline. CHIRAL ART Immobilized type offers excellent robustness for gradient analysis and highly sensitive analysis on LC/MS.
Gradient test | |
Column | 5 µm, 250 X 4.6 mmI.D. |
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Eluent | A) n-hexane B) ethanol 2-80%B (0-30 min) |
Flow rate | 1.0 mL/min |
Temperature | 25°C |
Detection | UV at 230 nm |
CHIRAL ART Immobilized type has excellent chemical stability and can be used across a wide pH range. The addition of acid/amine to the mobile phase or the use of buffer solution is an effective tool for improving peak shape and/or resolution.
Continuous flow of acid/alkaline solution | |
Column | 5 µm, 50 X 4.6 mmI.D. |
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Eluent | Buffer/methanol (90/10) |
Flow rate | 1.0 mL/min |
【Acidic condition】 | |
Buffer | 0.1% H3PO4 (pH 2) |
Temperature | 40°C |
【Basic condition】 | |
Buffer | 20 mM NH4HCO3-DEA (pH 9) |
Temperature | 25°C |
Column performance test | |
Column | 5 µm, 50 X 4.6 mmI.D. |
---|---|
Eluent | acetonitrile/water (30/70) for SB methanol/water (70/30) for SJ |
Flow rate | 1.0 mL/min |
Temperature | 25°C |
Detection | UV at 254 nm |
Sample | Benzoin for SB 1-(1-Naphthyl) ethanol for SJ |
On CHIRAL ART Immobilized type, the change in column performance after flushing with each solvent was less than 2%. Thus, these columns have high resistance to various solvents.
Amylose-SA | Cellulose-SB | Cellulose-SJ | ||||
---|---|---|---|---|---|---|
α | k'(2) | α | k'(2) | α | k'(2) | |
Ethyl acetate | 100.3% | 101.2% | 100.0% | 99.1% | 99.3% | 99.0% |
Tetrahydrofuran | 100.0% | 100.0% | 99.3% | 98.0% | 99.2% | 99.7% |
Dichloromethane | 100.3% | 100.6% | 101.3% | 99.6% | 99.6% | 98.4% |
Column | 5 µm, 50 X 4.6 mmI.D. |
---|---|
Eluent | n-hexane/2-propanol (95/5) |
Flow rate | 1.0 mL/min |
Temperature | 25°C |
Sample | Benzoin |