Columns
YMC-Triart metal-free (PEEK-lined) consists by a double-layered structure: PEEK-lined inner surface and stainless outer tube. YMC-Triart metal-free (PEEK-lined) is suitable for analysis of coordination compouns.
| Wetted part (liner) | PEEK |
|---|---|
| Outer tube | Stainless steel |
| Frit | PEEK |
Metal coordination compounds which have phosphate group in their structure tend to show poor peak shape by interacting with metal, such as stainless steel column. On nucleotides analysis shown below, better peak shape was obtained with the metal free column.
The metal free column is effective for highly-sensitive analysis using LC/MS, as well.
| Column | YMC-Triart C18 3 µm, 50 X 2.1 mmI.D. |
|---|---|
| Eluent | 5 mM HCOONH4 |
| Flow rate | 0.21 mL/min |
| Temperature | 25℃ |
| Detection | UV at 265 nm |
| Injection | 1 µL (10 µg/mL) |
Tubing or HPLC system components could induce peak shape deterioration. The example below shows the comparison of nucleotides analysis on an ordinary HPLC system and a “non-metal” HPLC system. The “non-metal” system consists of a PEEK sample loop, a PEEK injector needle seat and a PEEK tubing between injector and column. Just by using such PEEK parts, peak shape of coordination compounds can be greatly improved.
| Column | YMC-Triart C18 3 µm, 50 X 2.1 mmI.D. |
|---|---|
| Eluent | 5 mM HCOONH4 |
| Flow rate | 0.21 mL/min |
| Temperature | 25℃ |
| Detection | UV at 265 nm |
| Injection | 1 µL (10 µg/mL) |
