Columns
YMC-Actus series HPLC columns offer outstanding durability under high-speed gradient conditions. Desirable columns for laboratory scale preparative HPLC in R&D, etc. It is ideal for high-throughput purification in drug discovery.
YMC-Actus series have been developed by applying the Axial Compression Technology to semi-prep column (20 mm i. d. and 30 mm i. d.). The column bed is compressed adequately by attaching the end assembly newly designed for YMC-Actus. It provides proper bed density (10% higher than conventional columns) and bed uniformity. The combination of technology acquired by long our experience with DAC column, the advanced technique of slurry packing, and new hardware design offers an outstanding durability and efficiency for YMC-Actus.
*To Axial Compression Column
| Column | : | 50 X 20 mmI.D. 5 µm |
|---|---|---|
| Eluent | : | A) water B) methanol |
| Gradient | : | 5%B-95%B |
| Flow rate | : | 50 mL/min |
| Pressure | : | ∼17MPa |
In case of wrong packing conditions, rapid pressure change under the high-speed gradient condition degrades column performance. To examine bed stability, the column performance was evaluated after every 100 runs of the sequential high-speed gradient. YMC-Actus offers superior initial column efficiency compared to competitors. Furthermore, its excellent performance is maintained after a long series of gradient test.
| Column | : | 50 X 20 mmI.D. 5µm or 50 X 19 mmI.D. 5µm |
|---|---|---|
| Eluent | : | A) water B) methanol |
| Gradient | : | 5%B (0-0.5 min), 5-95%B (0.5-3.1 min),95%B (3.1-3.6 min), 5%B (3.6-4.0 min) |
| Flow rate | : | 50 mL/min |
| Column | : | 50 X 20 mmI.D. 5µm or 50 X 19 mmI.D. 5µm |
|---|---|---|
| Eluent | : | methanol/water (60/40) |
| Flow rate | : | 10 mL/min |
YMC-Actus provides outstanding stability and reproducibility in the separation of pharmaceuticals dissolved in 100% DMSO, even after 1000 injections under the fast gradient condition. It is ideal for high-throughput purification in drug discovery.
| Column | : | YMC-Actus Pro C18 RS (5 µm, 8 nm) 50 X 20 mmI.D. |
|---|---|---|
| Eluent | : | A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 10-90%B (0-3 min), 90%B (3-5 min), 10%B (5-10 min) |
| Flow rate | : | 20 mL/min |
| Temperature | : | ambient |
| Detection | : | UV at 270 nm |
| Injection | : | 100 µL (0.2-2 mg/mL in DMSO) |
| Packing material | Particle size (µm) |
Pore size (Å) |
C% | Usable pH range |
Characteristics | |
|---|---|---|---|---|---|---|
| Triart series | Triart C18 | 5 | 120 | 20 | 1 - 12 |
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| Triart C18 ExRS | 5 | 80 | 25 |
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| Triart C8 | 5 | 120 | 17 |
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| Triart Phenyl | 5 | 120 | 17 | 1 - 10 |
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| Triart PFP | 5 | 120 | 15 | 1 - 8 |
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| Triart Bio C18 | 5 | 300 | - | 1 - 12 |
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| Triart Bio C4 | 5 | 300 | - | 1 - 10 | ||
| Pro series | Pro C18 | 5 | 120 | 16 | 2 - 8 |
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| Hydrosphere C18 | 5 | 120 | 12 |
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| Pro C18 RS | 5 | 80 | 22 | 1 - 10 |
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| Pro C8 | 5 | 120 | 10 | 2 - 7.5 |
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| YMC-Pack series | ODS-A | 5 | 120 | 17 | 2 - 7.5 |
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| ODS-AQ | 5 | 120 | 14 |
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*Please contact us for packing materials other than those listed above
YMC-Actus has as high performance as the analytical column, and it is possible to scale up directly from analytical to preparative separation without loss in resolution.
Pro C18 RS has superior selectivity for hydrophobic compounds that differ slightly in structure and hydrophobicity. It achieved better resolution between nordihydrocapsaicin (peak 1) and capsaicin (peak 2) than competitors. Target compound was purified with high purity on YMC-Actus Pro C18 RS in direct scaling-up.
| Eluent | : | A) methanol/water/TFA (50/50/0.1) B) methanol/TFA (100/0.1) 10-30%B (0-5 min), 30%B (5-10 min) |
|---|---|---|
| Temperature | : | 25ºC for 50 X 4.6 mmI.D. ambient for 50 X 20 mmI.D. |
| Detection | : | UV at 280 nm |
| Sample | : | methanol extract of a commercial cayenne pepper (1 g cayenne pepper/3 mL) |
