YMC-Pack Polyamine II
YMC-Pack Polyamine II is a silica-based packing bonded with polyamine. It is particularly useful for separation of sugars. The column life of YMC-Pack Polyamine II in aqueous mobile phase is longer than conventional silica-based amino columns, and thus is applicable to separation of oligosaccharides using mobile phase with relatively higher water content. In addition, YMC-Pack Polyamine II can be used to separate ionic compounds with a combination of normal-phase mode and weak anion exchange mode.
| SPECIFICATION |
| Particle size |
5 µm |
| Pore size |
120 Å |
| Usable pH range |
2-7.5 |
- POINT
-
- Silica gel chemically bonded to polyamine
- The most suitable column for separation of sugars
- Solves durability problem of conventional silica-based amino columns
- Useful for separation of hydrophilic compounds including vitamins
- Useful for separation of fat-soluble compounds using nonaqueous mobile phase
- Features
-
Excellent durability
Even in a hard flow durability test using mobile phase that consists only of water, the retention time of sugars changes little.
Flow conditions
| Column |
YMC-Pack Polyamine II, 250 X 4.6 mmI.D. |
| Eluent |
water |
| Flow rate |
1.0 mL/min |
| Temperature |
ambient |
| Time |
100 hours |
Analytical conditions
| Eluent |
acetonitrile/water (75/25) |
| Flow rate |
1.0 mL/min |
| Temperature |
26°C |
| Detection |
RI, 32 X 10-6 RIU/FS |
The most suitable column for separation of sugars
- Fructose
- Glucose
- Sucrose
- Maltose
- Lactose
| Column |
YMC-Pack Polyamine II, 250 X 4.6 mmI.D. |
| Eluent |
acetonitrile/water (75/25) |
| Flow rate |
1.0 mL/min |
| Temperature |
26°C |
| Detection |
RI, 32 X 10-6 RIU/FS |
For normal-phase separation
YMC-Pack Polyamine II is applicable for separation of fat-soluble vitamins and water-soluble vitamins as a normal-phase column that can be used with water or buffer and various organic solvents.
| Column |
YMC-Pack Polyamine II, 250 X 4.6 mmI.D. |
| Eluent |
n-hexane/ethyl acetate (70/30) |
| Flow rate |
1.0 mL/min |
| Temperature |
30°C |
| Detection |
UV at 295 nm |
| Column |
YMC-Pack Polyamine II, 250 X 4.6 mmI.D. |
| Eluent |
50 mM NH4H2PO4/acetonitrile (30/70) |
| Flow rate |
1.0 mL/min |
| Temperature |
30°C |
| Detection |
UV at 250 nm |
