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BioPro IEX Q/S

BioPro IEX Q/S, which are based on a hydrophilic polymer with low nonspecific adsorption, are designed for capture and intermediate purification of proteins and nucleotides. High dynamic binding capacity (DBC) and high recovery allow fast large-scale purification processes. These media offers high productivity for industrial purification of peptides, proteins, and nucleotides, including biopharmaceuticals such as antibodies.

Specification

  BioPro IEX Q
 BioPro IEX S
Matrix Hydrophilic porous polymer
Particle size (µm) 75
Charged group -R-N+(CH3)3 -R-SO3-
Usable pH range 2-12
Ion-exchange capacity
(meq/mL-resin)		 >0.10
Dynamic binding capacity (DBC)
(mg/mL-resin)		 >160 (BSA) >160 (lysozyme)
Features

High dynamic binding capacity (DBC) and recovery

BioPro IEX Q/S have higher DBC for proteins than commercial ion exchange media. BioPro IEX Q/S are effective for protein purification during the capture step.

Anion exchanger Particle size
(µm)		 Ion exchange capacity
(meq/mL-resin)		 DBC*
(mg/mL-resin)
BioPro IEX Q75 75 0.13 183
Brand G (porous Q type) 90 0.19 102
Cation exchanger Particle size
(µm)		 Ion exchange capacity
(meq/mL-resin)		 DBC*
(mg/mL-resin)
BioPro IEX S75 75 0.12 192
Brand G (porous S type) 90 0.13 80
*Dynamic binding capacities were determined at 10% breakthrough under following conditions:
Column 50 X 4.6 mmI.D.
Linear velocity 3.0 cm/min (180 cm/hr)
for anion-exchange resin
Equilibration buffer 20 mM Tris-HCl (pH 8.6)
Elution buffer 0.5 M NaCl in equilibration buffer
Sample 1.5 mg/mL BSA in equilibration buffer
Detection UV at 280 nm
for cation-exchange resin
Equilibration buffer 20 mM Glycine-NaOH (pH 9.0)
Elution buffer 0.5 M NaCl in equilibration buffer
Sample 1.5 mg/mL Lysozyme in equilibration buffer
Detection UV at 300 nm

High productivity on purification

BioPro IEX Q/S show high DBC over a wide range of linear velocities, and the difference in DBC is less than 5% between 200 cm/hr and 1000 cm/hr. BioPro IEX Q/S give increased productivity and reduced cost in biopharmaceutical production.

Column 50 X 5.0 mmI.D.
Equilibration buffer 20 mM Glycine-NaOH (pH 9.0)
Elution buffer 0.5 M NaCl in equilibration buffer
Sample 1.0 mg/mL Lysozyme in equilibration buffer
Detection UV at 300 nm

Excellent durability under CIP

Cleaning in place (CIP) is an important procedure for cleaning and sterilizing columns used for protein purification. The DBC and the selectivity of proteins are unaffected following 20 cycles of CIP with 1 M NaOH. The high chemical stability of BioPro IEX Q/S allow effective cleaning with alkaline solution.


Conditions of DBC measurement
Column BioPro IEX S75, 50 X 5.0 mmI.D.
Linear velocity 800 cm/hr
Equilibration buffer 20 mM Glycine-NaOH (pH 9.0)
Elution buffer 0.5 M NaCl in equilibration buffer
Sample 1.0 mg/mL Lysozyme in equilibration buffer
Detection UV at 300 nm
*DBC is determined at 10% breakthrough

Suitable for capture step and intermediate purification step

Egg yolk antibody (IgY) can be isolated with high purity of more than 99% using two chromatographic purification steps, which consist of a capture step using ion exchange chromatography with BioPro IEX Q75 and a polishing step using size exclusion chromatography with YMC-Pack Diol-200.



Capture step by ion exchange chromatography (IEC)
BioPro IEX Q75, 75 µm, 50 X 4.6 mmI.D.



Eluent A) 20 mM Tris-HCl (pH 8.1)
B) 20 mM Tris-HCl (pH 8.1)
containing 0.5 M NaCl
10%B (0-15 min), 30%B (15-30 min), 90%B (30-40 min)
Flow rate 0.5 mL/min (180 cm/hr)
Temperature ambient
Detection UV at 280 nm
Injection 1 mL (ca. 20 mg Protein)
Sample Crude extract from egg yolk
(Courtesy of Pharma Foods International Co., Ltd.)


Polishing by size exclusion chromatography (SEC)
YMC-Pack Diol-200, 5 µm, 300 X 8.0 mmI.D.





Eluent 0.1 M KH2PO4-K2HPO4 (pH 6.9) containing 0.2 M NaCl
Flow rate 0.7 mL/min
Temperature ambient
Detection UV at 280 nm
Injection 1 mL (ca. 0.45 mg IgY)
Sample Fraction from capture step by IEC


Analysis of purified fraction
SEC (YMC-Pack Diol-200, 5 µm, 300 X 4.6 mmI.D.)



Non-reduced SDS-PAGE

Product list

Media

Packing material Particle size
(µm)		 Product number
BioPro IEX Q75 75 QAA0S75
BioPro IEX S75 SPA0S75

Screening Kit

Product name Particle size
(µm)		 Specification Column volume Product Number
BioPro IEX Q75 75 5/pack 1 mL BPQAA0S75-01PK
5 mL BPQAA0S75-05PK
BioPro IEX S75 1 mL BPSPA0S75-01PK
5 mL BPSPA0S75-05PK

To BioPro Ion Exchange Screening Kit

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